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Take a look at the step-by-step instructions on creating XY scatter graphs in Excel at these Websites:
Graphing with Excel: depts.noctrl.edu/biology/resource/handbook/xl.pdf downloaded from North Central College Biology Student HandbookGuide to fairly good graphs with Excel: http://udel.edu/~mcdonald/statgraph.html pages 274-286 in: McDonald, J.H. 2009. Handbook of Biological Statistics (2nd ed.). Sparky House Publishing, Baltimore, Maryland.
If you don't like Excel, try one of these free graphing software packages:
R - for statistical analysis and graphing: http://www.r-project.org/Gnuplot - graph oriented, with some computational capacities: http://www.gnuplot.info/GLE - graphics layout engine, allows much more than graphs: http://glx.sourceforge.net/
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